An investigation was carried out during the tenure of March–September 2014 in search of fungal diseases of sesame. Seeds of two varieties, namely, BARI Til 2 and local variety Assima were collected from Satkhira and Jamalpur districts. Seeds were sown at the experimental field plot of Botanic garden, Department of Botany, University of Dhaka. Three types of symptoms, namely, stem rot, leaf spot, and fruit rot were recorded on sesame before flowering up to harvesting. Aspergillus flavus, Aspergillus niger, Alternaria alternata, Fusarium sp., Lasiodiplodia theobromae (Botryodiplodia theobromae), Corynespora cassiicola, Curvularia lunata, Penicillium sp., and Sclerotium rolfsii were isolated from plant parts on PDA medium following tissue planting method at temperature 25–28°C and pH 6.0. Five fungicides, viz. Homai (Carbendazim), CM-75 (Carbendazim + Mancozeb), Ridomil Gold (Mancozeb + Matalexil), Score (Difenoconazole), and Tilt 250 EC (Propiconazole) at 50, 100, and 200 ppm concentration were used to control test pathogen S. rolfsii in vitro. Tilt 250 EC found most effective and completely inhibited the radial growth of test pathogen at all the concentrations used.